![](https://parts.igem.org/images/partbypart/icon_plasmid_backbone.png)
Plasmid_Backbone
Part:BBa_K4201014
Designed by: David Birkhaeuser Group: iGEM22_CU-Boulder (2022-10-11)
Chlor AarI
This Chloramphenicol Backbone conveys antibiotic resistance in transformed bacteria. The backbone also contains a Blue/White marker which is flanked by PaqCI and BsaI cut sites. This marker is excised during Golden Gate gene assembly and replaced by a gene of interest, allowing blue/white selection of transformed colonies. This backbone was used as an intermediate to be transformed into E. coli, before a gene of interest was inserted into an agrobacterium-ready pLSUK backbone.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 320
Illegal XbaI site found at 293
Illegal PstI site found at 281 - 12INCOMPATIBLE WITH RFC[12]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 320
Illegal PstI site found at 281 - 21INCOMPATIBLE WITH RFC[21]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 320
Illegal BamHI site found at 299 - 23INCOMPATIBLE WITH RFC[23]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 320
Illegal XbaI site found at 293
Illegal PstI site found at 281 - 25INCOMPATIBLE WITH RFC[25]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 320
Illegal XbaI site found at 293
Illegal PstI site found at 281 - 1000INCOMPATIBLE WITH RFC[1000]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal BsaI site found at 14
Illegal BsaI.rc site found at 736
[edit]
Categories
Parameters
None |